Document Details
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Document Type |
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Article In Journal |
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Document Title |
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CYTOTOXIC ACTIVITY OF BIOACTIVE FRACTIONS FROM PM 701. السامة للخلايا النشاط الكسور النشطة بيولوجيا من الساعة 701. |
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Subject |
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CYTOTOXIC ACTIVITY OF BIOACTIVE FRACTIONS FROM PM 701. |
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Document Language |
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English |
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Abstract |
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The cytotoxic activity of PM 701 (a clean and sterile natural product) and fractions
obtained thereof was assessed against human cancer cell lines, namely, hepatocellular
carcinoma (HEPG2), colon carcinoma (HCT 116) and human glioma (U251) cells by
using sulforhodamine B assay. PM 701 was extracted with methyl alcohol to give PMF
fraction that was chromatographed on a Si gel column to yield seven fractions (frs: M2-
M8). PMF and its sub-fractions demonstrated a variable and notably high cytotoxic
activity against the tested cell lines (IC50 0.5-10 μg/mL). Fractions M2 and M5 were
found to demonstrate the most potent activity against HEPG2 cell line (IC50 0.54 μg/mL
for each), followed by fraction M8 (IC50 1.01 μg/mL). The effect of the different
fractions against normal human foreskin cell line (HFS) was also evaluated. Isolation and
identification of the active cytotoxic compounds are now in progress in our laboratories |
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ISSN |
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1579-4377 |
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Journal Name |
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Electronic Journal of Environmental, Agricultural and Food Chemistry |
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Volume |
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8 |
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Issue Number |
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11 |
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Publishing Year |
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1429 AH
2009 AD |
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Article Type |
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Article |
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Added Date |
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Saturday, March 5, 2011 |
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Researchers
| فاتن خورشيد | Khorshid, fathen | Researcher | Doctorate | |
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